The Gold Standard in Creatine Testing for Pectin Based Gummies
We use a modified USP Creatine Monograph test. This test is considered the “Gold Standard” for testing pectin-based gummies. There are two, more common, off-the-shelf creatine tests that are widely used methods for testing creatine, but neither is suitable for pectin-based gummies:
- Analytical Biochemistry (1993) HPLC Method – Originally developed for measuring creatine in biological tissues (e.g., muscle samples), this method does not account for the unique structure of pectin-based gummies, leading to incomplete extraction and inaccurate results.
- USP Creatine Monograph (HPLC-Based) – Considered the gold standard for creatine powders and capsules, but it has limitations when applied to gummies, including:
- Incomplete extraction – Pectin's gel-like nature can trap creatine, skewing results.
- Filtration issues – High viscosity can interfere with the separation process, affecting HPLC accuracy.
- Organic acid interference – Ingredients like citric acid can co-elute with creatine, making detection unreliable.
How We Ensure Accurate Testing
Because of these challenges, neither of these standard tests will provide reliable results for pectin-based gummies. That’s why we work with Eurofins, a leading third-party lab, which has developed a modified version of the USP Creatine Monograph specifically for pectin based gummies. This adaptation ensures:
✅ Full creatine extraction from the gummy matrix
✅ Proper filtration to prevent interference
✅ Accurate quantification despite pectin-based challenges
It’s important to note that most labs, including Eurofins, do not automatically use this modified method unless specifically requested. This is why some third-party tests may incorrectly report lower creatine levels.